Table III.
SMT2 enzyme assay, sterol profile, and plant size in T2 generations of p35S-SMT2 lines
| Line/No. of Transgenic Locia | Individual/Genetic Structureb | SMT2 Enzyme Activity | R
|
Plant Height | |
|---|---|---|---|---|---|
| Microsomes | Total sterols | ||||
| nmol mg−1 protein h−1 | cm | ||||
| Wild typec | – | 0.88 ± 0.33 | 0.99 ± 0.07 | 1.00 ± 0.03 (1.35 ± 0.20) | 59 ± 2 (142 ± 11) |
| p35Sc | – | 0.80 ± 0.30 | 0.90 ± 0.06 | 0.75 ± 0.06 (0.95 ± 0.34) | 60 ± 1 (146 ± 15) |
| A/1 | 1/hom | 5.9d | 0.05 | 0.02 (0.04) | 39 (64) |
| 2/hom | 3.2 | 0.09 | 0.13 (0.10) | 56 (135) | |
| 3/hom | 7.5 | 0.03 | 0.02 (0.04) | 54 (120) | |
| 4/het | 4.1 | 0.17 | 0.14 (0.17) | 63 (145) | |
| B/2 | 1/het | 3.9 | 0.14 | 0.15 (0.18) | 56 (130) |
| 2/het | 5.4 | 0.04 | 0.04 (0.02) | 42 (85) | |
| 3/hom | 2.8 | 0.07 | 0.10 (0.08) | 53 (130) | |
| 4/het | 8.0 | 0.03 | 0.02 (0.04) | 45 (110) | |
| C/1 | 1/het | 1.8 | 0.16 | 0.18 (0.18) | 53 (135) |
| 2/hom | 5.4 | 0.09 | 0.09 (0.14) | 48 (130) | |
| 3/het | 3.7 | 0.17 | 0.18 (0.19) | 50 (135) | |
| 4/− | 3.0 | 0.17 | 0.18 (0.20) | 58 (130) | |
| D/2 | 1/het | 5.7 | 0.03 | 0.04 (0.07) | 48 (120) |
| 2/het | 4.6 | 0.03 | 0.02 (0.08) | 51 (110) | |
| 3/het | 6.3 | 0.03 | 0.05 (0.04) | 39 (90) | |
| 4/het | 10.5 | 0.03 | 0.03 (0.03) | 28 (55) | |
| E/1 | 1/het | 3.7 | 0.15 | 0.15 (0.20) | 59 (130) |
| 2/het | 3.6 | 0.15 | 0.16 (0.17) | 56 (125) | |
| 3/hom | 12.6 | 0.03 | 0.05 (0.04) | 38 (90) | |
| 4/hom | 11.0 | 0.03 | 0.02 (0.04) | 36 (85) | |
Values in parentheses are data collected from the same plants after seed setting.
Primary transformants were selfed and the resulting T2 progenies were segregated for kanamycin resistance on a germination medium. Sample sizes ranged between 50 and 140 individuals. Hypotheses of the number of mendelian loci were challenged according to χ2 tests (data not shown).
T2 individuals were selfed and the resulting T3 progenies were scored for kanamycin resistance as described in footnote a. hom, Homozygote; het, heterozygote; –, not done.
Values presented for wild-type and transgenic controls are means ± sd of four individuals.
Mean value of two experiments; sd never exceeded 30%.