Hepa1–6 cells are treated with SAMe (A–D), Betaine(E–H) or combination of Betaine and SAMe(I–L). Photomicrographs taken four days after treatment. SAMe reduced cell proliferation and induced cell death in dose dependent manner (A: control, B: SAMe 1mM, C: SAMe 2mM, D : SAMe 4mM). Betaine itself had no significant effect on cell proliferation and survival(E : control, F: betaine 1%, G betaine 2%, H betaine 4%), however, it attenuates SAMe’s effect (I :control, J:SAMe 1mM and betaine 1%, K : SAMe 2mM and betaine 2%, L: SAMe 4mM and betaine 4%)