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. 2012 Oct 26;7(10):e47629. doi: 10.1371/journal.pone.0047629

Figure 2. Genome wide transcriptome analysis of rMAPC-SMC differentiation.

Figure 2

rMAPCs (Cl19 and 3c3) were cultured for 6 days with TGFβ1 and PDGF-BB in serum free medium, and RNA harvested from triplicate samples on days 0, 2, 4 and 6. In addition, RNA from cultured RAOSMCs was obtained in duplicate. A. Ingenuity pathway analysis (IPA) of the differentially expressed genes in both clones of rMAPCs identified predominantly developmental and cardiaovascular system associated genes (table represents data obtained as output from IPA). B. Pathways relating to cardiogenesis were among the top canonical pathways (table represents data obtained as output from IPA). C. Validation by PCR of the target TF genes identified by microarray analysis.