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. 2012 Oct 26;7(10):e47629. doi: 10.1371/journal.pone.0047629

Figure 3. Knock down of Baf60c down regulates αSMA protein levels.

Figure 3

rMAPCs were transduced with a doxycyclin (dox) inducible anti-Baf60c shRNA containing lentiviral vector or a vector containing a scrambled shRNA and subjected to SMC differentiation using TGFβ1. A. Quantitative RT-PCR for Baf60c mRNA levels in cells transduced with anti-Baf60c shRNAs in presence (+Dox) or absence (−Dox) of doxycycline up on TGFβ1 stimulation (Mean±s.e.m of n = 5; Student's t-test p<0.05). B. Western blots for Baf60c, αSMA, SM22α expression in cells transduced with anti-Baf60c shRNAs in the presence (+) or absence (−) of doxycyclin in rMAPC treated with TGF-β. C. Western blots for BAF60c and SM22α in RAOSMC treated with either siRNA against Baf60c or control.