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. 2012 Oct 26;7(10):e47629. doi: 10.1371/journal.pone.0047629

Figure 4. Baf60c is sufficient to induce smooth muscle like fate in rMAPCs.

Figure 4

rMAPCs were transduced with an inducible Baf60c and eGFP cDNA encoding lentiviral vector or a vector encoding only eGFP, and subjected to SMCs without exogenous TGFβ1, and differentiation to SMCs examined by RT-qPCR and immunostaining. A. Expression of smooth muscle specific mRNAs compared to undifferentiated rMAPC (expression normalized to GAPDH) (Mean±s.e.m of n = 4; p<0.05). B. The expression of αSma in Baf60c transduced cells on day 9 cells is significantly higher compared to GFP transduced control cells (Mean±s.e.m of n = 4; Student's t-test p<0.05). C. Baf60c overexpression is sufficient to induce smooth muscle like fate with cells expressing mature smooth muscle proteins as compared to GFP transduced cells (smooth muscle proteins are colored pseudo green).