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. 2012 Oct 26;7(10):e48204. doi: 10.1371/journal.pone.0048204

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© 2012 Soppina et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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COS7 cells expressing the deacetylases mCit-HDAC6 or mCit-SIRT2 (green) were fixed and double stained using A) monoclonal 6-11B-1 (red) and total tubulin (magenta) antibodies or B) polyclonal anti-acetyl-K40 (red) and total tubulin (magenta) antibodies. Transfected cells are indicated by the yellow dotted outline. Scale bars, 20 µm.