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. 1998 Oct;118(2):471–482. doi: 10.1104/pp.118.2.471

Figure 1.

Figure 1

RNA gel blots of total leaf RNA prepared from Lggs and Lgsh, which were transformed using the T-DNA constructs shown below the blots. A, Lggs. The probe was an EcoRI internal, 1.4-kb fragment of the gshI-coding sequence. All lanes were loaded with 20 μg of total foliar RNA. The 18S probe corresponded to a 0.5-kb fragment of a cDNA for the 18S radish rRNA. B, Lgsh. The probe was an MslI internal, 0.9-kb fragment of the gshII-coding sequence. RNA loaded and loading control are as in A. WT, Wild type. C, T-DNA construct for overexpression of γ-ECS in the chloroplast. P70, Cauliflower mosaic virus 35S promoter with double-enhancer sequence; L, pea rbcS sequence coding for the transit peptide; T, cauliflower mosaic virus poly(A) sequence; LB, left border; RB, right border; nos, nopaline synthase; nptII, neomycin phosphotransferase. D, T-DNA construct for overexpression of GS in the chloroplast. Other abbreviations are as in C.