Figure 4.

Confirmation of the intracellular location of the bacterial gene products in ggs, Lggs, gsh, and Lgsh. Lanes L, Leaf extract prepared as for assay of extractable foliar enzyme activities; lanes F, chloroplast filtrate prior to centrifugation; lanes C, resuspended chloroplast pellet. WT, Untransformed (wild-type) poplar. Gels were 12% acrylamide. All lanes were loaded with 5 μg of total soluble protein. A, Immunoblot using antiserum raised against the E. coli γ-ECS. B, Immunoblot using antiserum raised against the E. coli GS.