Figure 6. The effect of TNFα on GPDH activity and gene expression of large yellow croaker adipocytes during differentiation.

The confluent cells were exposed to the differentiation medium without the lipid mixture but supplemented with 0, 1, 10 or 100 ng/ml hrTNFα for 6 days and evaluated by GPDH, the indicator of cell differentiation. The gene expression levels were determined by quantitative Real-Time PCR. Data were analyzed by using 2^−ΔΔCt [87] and are referred to the control treatment (TNFα = 0) using β-actin as a control. Data are means ± SEM, n = 3. Different letters indicate significant differences at P<0.05. ATGL =  adipose triglyceride lipase, FAS = fatty acid synthase, LPL = lipoprotein lipase, PPAR  = proliferators-activated receptor α, γ.