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. 2012 Oct 26;7(10):e46427. doi: 10.1371/journal.pone.0046427

Figure 1. Nedd4-1 protein is absent from MyoCre;Nedd4-1flox/flox (Nedd4-1 SMS-KO) skeletal muscle.

Figure 1

(A) SDS-PAGE and Nedd4-1 Western blotting (WB) of 50 µg of gastrocnemius muscle lysates from representative Nedd4-1 SMS-KO mice (M4, M5, M6) and littermate MyoCre;Nedd4-1+/+ control mice (M1, M2, M3) reveal the absence of Nedd4-1 in the Nedd4-1 SMS-KO muscle. Nedd4-2 protein remains equally expressed in the muscle of Nedd4-1 SMS-KO and MyoCre;Nedd4-1+/+ control mice. GAPDH served as a loading control. (B) WB of heart, liver and kidney protein lysates reveal equal Nedd4-1 protein in these tissues in the Nedd4-1 SMS-KO and littermate MyoCre;Nedd4-1+/+ control mice. GAPDH served as a loading control for all blots; liver lysate is shown as a representative blot.