Figure 3. Subcellular localization of OatA^TM10-YFP and OatB^TM10-YFP fusions.

A) Schematic representation of fusion proteins (YFP, yellow star) and corresponding micrographs in phase contrast (PC) microscopy and fluorescence microscopy (YFP). Upper panels, oatA mutant producing cytoplasmic YFP (OatA^−/YFP) used as control; middle panels, oatA mutant producing OatA^TM1–10-YFP (OatA^−/OatA^TM1–10-YFP); lower panels, oatB mutant producing OatB^TM1–10-YFP (OatB^−/OatB^TM1–10-YFP). Induction of expression was performed with 10 ng/ml of nisin. Bar scale, 2.0 µm. B) Fluorescence ratio (FR; AU, arbitrary unit) between the fluorescence measured at mid-cell position and pole. A^−/A™, oatA mutant producing OatA^TM1–10-YFP and B^−/B™, oatB mutant producing OatB^TM1–10-YFP. Lines represent the mean value (n = 20, 3 independent replicates).