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. 2012 Oct 26;7(10):e45899. doi: 10.1371/journal.pone.0045899

Table 2. qPCR results for ten selected differentially expressed genes.

Gene Sequence 5′-3′ R2 Efficiency (%) Amplicon size pb Ratio Stress vs Control p-value
HeAn_C_266 Forward CCATCGAACTAAGGCCACATReverse CACGCAAAGCTCCAACATAA 0.999 91 166 7.05 0.0326
HeAn_C_3312 Forward TTCTTCCCCACCCTTTTTCTReverse GAGGTTCTGATCGGTGTGGT 1.000 88 143 2.04 0.0518
HeAn_C_5545 Forward CCGGAAATCGTTGTTCAAGTReverse TCAGTGACACGAACGAGACC 0.998 93 164 4.79 0.0344
HeAn_C_2759 Forward CCCGAGTTGCAAAAAGTTGTReverse CCCTTCATTTGCATTGCTTT 0.999 87 135 2.67 0.0236
HeAn_C_9326 Forward AACCCAAGTTTGATCCATGCReverse GGTCAGCCACCTCACGTAAT 0.993 100 119 1.12 0.3721
HeAn_C_593 Forward GCGACAGAAGAAAAGGCAACReverse TGCAACCAGATCTGAAGACG 1.000 88 167 3.38 0.0412
HeAn_C_1482 Forward AGCCGTTACATCCCCTCTTTReverse ACAACCCGGGGATTCTACTC 0.999 87 122 4.7 0.043
HeAn_C_4439 Forward GGAAACATAGGTTGCGAGGAReverse CCTTTGACCCGTCTTTTCAA 1.000 86 101 3.51 0.0292
HeAn_C_2975 Forward ATCGACATCCCACACAGTGAReverse AACATGCCCACCGTAAATGT 0.997 96 102 2.10 0.0331
HeAn_C_4809 Forward GACGTTGAACGGGTCTTGTTReverse TGAAGCAACGCCTGATTATG 0.999 88 164 2.82 0.0222