Table 1. Design of experiment for understanding the toxicity of dextran-cerium oxide nanoparticles on E. coli and the observed toxicity in each condition.
| x1 | x2 | x3 | x4 | x5 | x6 | x7 | x8 | x9 | ||
| pH | [MgSO4] | [KCl] | [CaCl2] | [NOM] | [nano] | [E. coli] | RPM | [Fructose] | Toxicity | |
| Variable | ppm | ppm | ppm | ppm | ppm | cells/mL | ppm | % | ||
| Minimum | 6 | 0 | 0 | 0 | 0 | 0 | 4.63E+05 | 0 | 0 | |
| Maximum | 8 | 15 | 15 | 15 | 3 | 4.3 | 4.68E+07 | 400 | 100 | |
| Experiment | ||||||||||
| 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0.12 | 0 | 0 | 21 |
| 2 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.61 | 0.5 | 0.5 | 37 |
| 3 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.60 | 1.5 | 1.5 | 43 |
| 4 | 2 | 2 | 2 | 2 | 2 | 2 | 2.10 | 2 | 2 | 33 |
| 5 | 0 | 2 | 2 | 2 | 2 | 2 | 2.10 | 2 | 2 | 39 |
| 6 | 2 | 0 | 2 | 2 | 2 | 2 | 2.10 | 2 | 2 | 38 |
| 7 | 2 | 2 | 2 | 0 | 2 | 2 | 2.10 | 2 | 2 | 0 |
| 8 | 2 | 2 | 2 | 2 | 0 | 2 | 2.10 | 2 | 2 | 13 |
| 9 | 2 | 2 | 2 | 2 | 2 | 2 | 0.10 | 2 | 2 | 21 |
| 10 | 2 | 2 | 2 | 2 | 2 | 2 | 2.10 | 2 | 0 | 29 |
| 11 | 0.5 | 1 | 1 | 1 | 1 | 1 | 1.11 | 1 | 1 | 13 |
| 12 | 1 | 0.5 | 1 | 1 | 1 | 1 | 1.11 | 1 | 1 | 67 |
| 13 | 1 | 1 | 0.5 | 1 | 1 | 1 | 1.11 | 1 | 1 | 0 |
| 14 | 1 | 1 | 1 | 0.5 | 1 | 1 | 1.11 | 1 | 1 | 4 |
| 15 | 1 | 1 | 1 | 1 | 0.5 | 1 | 1.11 | 1 | 1 | 24 |
| 16 | 1 | 1 | 1 | 1 | 1 | 0.5 | 1.11 | 1 | 1 | 41 |
| 17 | 1 | 1 | 1 | 1 | 1 | 1 | 0.61 | 1 | 1 | 0 |
| 18 | 1 | 1 | 1 | 1 | 1 | 1 | 1.11 | 0.5 | 1 | 99 |
| 19 | 1 | 1 | 1 | 1 | 1 | 1 | 1.11 | 1 | 0.5 | 22 |
| 20 | 1.5 | 1 | 1 | 1 | 1 | 1 | 0.72 | 1 | 1 | 24 |
| 21 | 1 | 1.5 | 1 | 1 | 1 | 1 | 0.72 | 1 | 1 | 4 |
| 22 | 1 | 1 | 1.5 | 1 | 1 | 1 | 0.72 | 1 | 1 | 0 |
| 23 | 1 | 1 | 1 | 1.5 | 1 | 1 | 0.72 | 1 | 1 | 3 |
| 24 | 1 | 1 | 1 | 1 | 1.5 | 1 | 0.72 | 1 | 1 | 0 |
| 25 | 1 | 1 | 1 | 1 | 1 | 1.5 | 0.72 | 1 | 1 | 0 |
| 26 | 1 | 1 | 1 | 1 | 1 | 1 | 1.02 | 1 | 1 | 17 |
| 27 | 1 | 1 | 1 | 1 | 1 | 1 | 0.72 | 1.5 | 1 | 0 |
| 28 | 1 | 1 | 1 | 1 | 1 | 1 | 0.72 | 1 | 1.5 | 19 |
| 29 | 1.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.39 | 0.5 | 0.5 | 31 |
| 30 | 0.5 | 1.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.39 | 0.5 | 0.5 | 30 |
| 31 | 0.5 | 0.5 | 1.5 | 0.5 | 0.5 | 0.5 | 0.39 | 0.5 | 0.5 | 12 |
| 32 | 0.5 | 0.5 | 0.5 | 1.5 | 0.5 | 0.5 | 0.39 | 0.5 | 0.5 | 8 |
| 33 | 0.5 | 0.5 | 0.5 | 0.5 | 1.5 | 0.5 | 0.39 | 0.5 | 0.5 | 0 |
| 34 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 1.5 | 0.39 | 0.5 | 0.5 | 17 |
| 35 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.94 | 0.5 | 0.5 | 2 |
| 36 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.39 | 1.5 | 0.5 | 8 |
| 37 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.39 | 0.5 | 1.5 | 11 |
| 38 | 0 | 2 | 2 | 2 | 2 | 0 | 0.11 | 2 | 2 | 13 |
| 39 | 2 | 0 | 2 | 2 | 2 | 0 | 0.11 | 2 | 2 | 14 |
| 40 | 2 | 2 | 0 | 2 | 2 | 0 | 0.11 | 2 | 2 | 44 |
| 41 | 0 | 0 | 0 | 2 | 0 | 0 | 0.11 | 0 | 0 | 20 |
| 42 | 2 | 2 | 2 | 2 | 0 | 0 | 0.11 | 2 | 2 | 20 |
| 43 | 2 | 2 | 2 | 2 | 2 | 0 | 0.11 | 2 | 2 | 21 |
| 44 | 0 | 0 | 0 | 0 | 0 | 0 | 0.83 | 0 | 0 | 29 |
| 45 | 2 | 2 | 2 | 2 | 2 | 0 | 0.11 | 0 | 2 | 7 |
| 46 | 2 | 2 | 2 | 2 | 2 | 0 | 0.11 | 2 | 0 | 29 |
| 47 | 1 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 0.59 | 1.5 | 1.5 | 7 |
| 48 | 1.5 | 1 | 1.5 | 1.5 | 1.5 | 1.5 | 0.59 | 1.5 | 1.5 | 18 |
| 49 | 1.5 | 1.5 | 1 | 1.5 | 1.5 | 1.5 | 0.59 | 1.5 | 1.5 | 18 |
| 50 | 1.5 | 1.5 | 1.5 | 1 | 1.5 | 1.5 | 0.59 | 1.5 | 1.5 | 0 |
| 51 | 1.5 | 1.5 | 1.5 | 1.5 | 1 | 1.5 | 0.59 | 1.5 | 1.5 | 0 |
| 52 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1 | 0.59 | 1.5 | 1.5 | 0 |
| 53 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 0.65 | 1.5 | 1.5 | 0 |
| 54 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 0.59 | 1 | 1.5 | 0 |
| 55 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 0.59 | 1.5 | 1 | 0 |
| 56 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 0.28 | 0.5 | 0.5 | 19 |
| 57 | 1 | 1 | 1 | 1 | 1 | 0 | 0.45 | 1 | 1 | 22 |
| 58 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 0 | 0.62 | 1.5 | 1.5 | 20 |
| 59 | 2 | 2 | 2 | 2 | 2 | 0 | 0.79 | 2 | 2 | 51 |
| 60 | 0.5 | 1 | 1 | 1 | 1 | 0 | 0.45 | 1 | 1 | 7 |
| 61 | 1 | 0.5 | 1 | 1 | 1 | 0 | 0.45 | 1 | 1 | 15 |
| 62 | 1 | 1 | 0.5 | 1 | 1 | 0 | 0.45 | 1 | 1 | 0 |
| 63 | 1 | 1 | 1 | 0.5 | 1 | 0 | 0.45 | 1 | 1 | 44 |
| 64 | 1 | 1 | 1 | 1 | 0.5 | 0 | 0.45 | 1 | 1 | 25 |
| 65 | 1 | 1 | 1 | 1 | 1 | 0 | 0.45 | 1 | 1 | 31 |
| 66 | 1 | 1 | 1 | 1 | 1 | 0 | 0.28 | 1 | 1 | 13 |
| 67 | 1 | 1 | 1 | 1 | 1 | 0 | 0.45 | 1 | 0.5 | 23 |
| 68 | 1 | 1 | 1 | 1 | 1 | 0 | 0.45 | 0.5 | 1 | 30 |
| 69 | 0 | 0 | 2 | 0 | 2 | 2 | 1.07 | 2 | 0 | 27 |
| 70 | 0 | 0 | 2 | 0 | 2 | 0 | 1.07 | 2 | 0 | 29 |
| 71 | 0 | 0 | 0 | 0 | 2 | 0 | 1.07 | 2 | 0 | 24 |
| 72 | 2 | 2 | 0 | 0 | 1 | 0 | 0.32 | 0 | 1 | 64 |
| 73 | 2 | 0 | 0 | 2 | 0 | 0 | 0.32 | 0 | 2 | 62 |