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. 2012 Oct 26;7(10):e48034. doi: 10.1371/journal.pone.0048034

Figure 8. Astrocyte aging does not affect the colocalization of bassoon and VGLUT 1.

Figure 8

(A) Representative images illustrating colocalization of the presynaptic proteins bassoon (green) and VGLUT 1 (red). Autaptic neurons were co-cultured with 5- (control), 9- and 16-week-old astrocytes. (B) The number of bassoon-positive puncta in autaptic neurons co-cultured with 5- (control), 9- and 16-week-old astrocytes (n = 30, 29 and 35 neurons, respectively). (C) The number of VGLUT 1-positive puncta in autaptic neurons. Data in (C-E) were obtained from the neurons analyzed in (B). (D) Percentage of bassoon-positive puncta that overlapped with VGLUT 1-positive puncta. Data were analyzed by assigning regions of interest in the bassoon images and transferring them to VGLUT 1 images of the same neurons. (E) Percentage of VGLUT 1 puncta that overlapped with bassoon puncta. Data were analyzed by assigning regions of interest in the VGLUT 1 images and transferring them to the bassoon images of the same neurons.