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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Behav Brain Res. 2012 Aug 20;236(1):35–47. doi: 10.1016/j.bbr.2012.08.020

Figure 6. Histological staining of sciatic nerves.

Figure 6

(a) Sciatic nerves from BMSC-treated WT, BMSC-treated twitcher, and untreated twitcher mice were obtained after euthanasia at PND37 and processed for histological staining using Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and Periodic Acid-Schiff (PAS) stains. The scale bar represents 50 microns for all larger images (10X), and each smaller image (40X) is a fourfold magnification of the larger image. Comparisons of the HE, LFB, and PAS images of the twitcher mouse sciatic nerves show a decrease in the number of apoptotic cells (white arrows) and infiltrating immune cells (black arrows) in the PNS after IP administration of BMSCs. (b) The number of infiltrating immune cells in the HE stains of the WT-BMSC (N=5), BMSC (N=4), and Twi (N=8) groups were calculated, compared to the total pixels for each scanned image, and normalized to the WT-BMSC group. (c) The staining intensity in the LFB stains of the WT (n=3), BMSC (n=4), and Twi (n=7) groups were calculated, compared to the total pixels for each scanned image, and normalized to the WT-BMSC group. Following one-way ANOVA, comparisons were made using Bonferroni’s post-hoc testing. Significant differences are denoted by *P<0.05; **P<0.01; ***P<0.001 vs. WT-BMSC and #P<0.05 vs. Twi mice.