Table 2. GLD mouse genotype and treatment-related differences in physical characteristics, computer-automated spatial and arena parameters, and manually observed behavior during PND23–29.
Variables calculated from tracks using EthoVision XT7 and manual behavior observations are provided for BMSC-treated WT (WT-BMSC), BMSC-treated twitcher (BMSC), HBSS-injected twitcher (HBSS), and untreated twitcher (Twi) mice. The ratio of males (M) to females (F) is provided for each group as (M/F). Oneway ANOVA was performed for the four mouse groups, and comparisons between the groups were made using Bonferroni’s post-hoc testing.
| WT-BMSC (Galc+/+) | BMSC (Galctwi/twi) | HBSS (Galctwi/twi) | Twi ((Galctwi/twi)) | ||||
|---|---|---|---|---|---|---|---|
| Mean±SEM | Mean±SEM | BMSC v. WT (*) BMSCv. HBSS(†) BMSC v. Twi (#) |
Mean±SEM | HBSS v. WT (*) | Mean±SEM | Twi v. WT (*) | |
| Characteristics | |||||||
| Average age (PND) | 25.20±0.35 | 25.60±0.50 | 26.52±0.14 | 26.40±0.53 | |||
| Average weight (g), PND 25 | 11.78±0.31 | 8.46±0.32 | *** | 9.31±0.17 | *** | 9.10±0.31 | *** |
| Spatial Parameters | |||||||
| Number of animals (M/F) | 15(8/7) | 13 (6/7) | 19(9/10) | 19(10/9) | |||
| Total distance traveled (m) | 5.30±0.42 | 4.48±0.29 | 3.67±0.27 | ** | 3.58±0.27 | ** | |
| Average velocity (cm/s) | 8.86±0.72 | 7.47±0.48 | 6.11±0.44 | ** | 5.97±0.45 | ** | |
| Maximum velocity (cm/s) | 25.65±3.32 | 18.43±1.80 | 15.05±2.57 | * | 14.82±2.55 | * | |
| Angular velocity (°/s) | 1996±49.54 | 2103±55.40 | ###, †† | 2392±54.19 | *** | 2421±51.31 | *** |
| Mean turn angle (°) | 66.54±1.65 | 70.10±1.85 | ###, †† | 79.82±1.81 | *** | 80.70±1.71 | *** |
| Average moving duration (s) | 17.04±3.40 | 10.09±1.79 | 5.33±1.03 | *** | 6.33±1.36 | ** | |
| Moving frequency | 1.50±0.14 | 1.08±0.08 | 0.83±0.12 | *** | 0.83±0.10 | *** | |
| Average time immobile (s) | 54.47±10.77 | 73.34±12.66 | # | 131.6±15.18 | ** | 133.30±17.03 | ** |
| Inner zone frequency | 54.73±6.03 | 38.54±5.47 | †† | 12.47±2.04 | *** | 23.84±6.08 | *** |
| Inner zone duration (s) | 105.10±9.83 | 54.54±9.67 | 46.71±12.66 | ** | 56.01±13.97 | * | |
| Behavioral Endpoints | |||||||
| Number of animals (M/F) | 11 (4/7) | 13 (6/7) | 19(9/10) | 12(7/5) | |||
| Latency to groom (s) | 33.20±6.62 | 39.75±11.71 | 61.60±22.45 | 65.47±15.34 | |||
| Paw lick duration (s) | 14.27±1.53 | 22.13±4.96 | 21.05±4.13 | 19.50±3.96 | |||
| Body/leg wash duration (s) | 7.46±1.82 | 5.50±1.76 | 5.32±2.24 | 5.00±2.43 | |||
| Number of unprotected rears | 5.36±0.88 | 0.38±0.18 | *** | 0.79±0.36 | *** | 0.25±0.18 | *** |
| Number of protected rears | 15.00±2.44 | 9.13±1.68 | 3.63±0.92 | *** | 4.00±1.64 | *** | |
| Number of total rears | 20.36±3.25 | 9.50±1.68 | ** | 2.61±0.66 | *** | 3.33±1.32 | *** |
| Number of defecations | 3.91±0.59 | 1.50±0.42 | ** | 1.05±0.22 | *** | 1.67±0.43 | ** |
| Number of digs | 1.43±1.13 | 9.50±1.76 | ***, ###, ††† | 0.00 | 0.00 | ||
*
P<0.05;
**
P<0.01;
***
P<0.001 vs. WT-BMSC.
#
P<0.05;
##
P<0.01;
###
P<0.001 vs. Twi.
†
P<0.05;
††
P<0.01;
†††
P<0.001 vs. HBSS.