Fig. 1.

Differential accumulation of Plins in cells cultured in the absence or presence of fatty acid and/or cholesterol. (A) Y1 adrenal cells were cultured overnight in the absence or presence of oleic acid and/or cholesterol. Whole cell lysates were prepared and endogenous Plins assayed by immunoblotting. Data are representative of three experiments. (B) McARH7777 rat liver cells were transiently transfected with the indicated GFP–Plin-expressing constructs and cultured overnight in the absence or presence of oleic acid and/or cholesterol. Whole cell lysates were prepared and GFP–Plin assayed by immunoblotting. Data are representative of three experiments.