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. 2012 Sep 1;125(17):4077–4089. doi: 10.1242/jcs.104950

Fig. 3.

Fig. 3.

Binding of CAS-1 to G-actin and the UNC-60B–actin complex, examined by nondenaturing polyacrylamide gel electrophoresis. Various concentrations (1–10 µM) of MBP (A), MBP–CAS-1 (B), MBP–CAS-1N (C) or MBP–CAS-1C (D) were incubated with buffer only (lanes 4–7) or buffer with 10 µM G-actin (lanes 8–11) or 10 µM G-actin and 10 µM UNC-60B (lanes 12–15) and examined by nondenaturing acrylamide gel electrophoresis. G-actin alone (lane 1), UNC-60B alone (lane 2), and mixtures of G-actin and UNC-60B (lane 3) were also applied to determine positions of free G-actin, free UNC-60B and the actin–UNC-60B complex (asterisks).