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. 2012 Sep 1;125(17):4077–4089. doi: 10.1242/jcs.104950

Fig. 5.

Fig. 5.

Effects of CAS-1 and UNC-60B on actin filament turnover as determined by the rate of phosphate release. Final 5 µM F-actin was mixed with various concentrations of UNC-60B and MBP–CAS-1 (A), MBP–CAS-1N (B) or MBP–CAS-1C (C), and the rate of Pi release (arbitrary units) was determined and plotted as a function of concentrations of UNC-60B. (D) Rate of Pi release from 5 µM F-actin with 5 µM UNC-60B in the presence of 0–0.5 µM MBP–CAS-1 (circles), MBP–CAS-1N (triangles) or MBP–CAS-1C (squares) were plotted as a function of the concentrations of the CAS-1 variants. Data are means ± s.d. of three independent experiments.