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. 2012 Sep 1;125(17):4090–4102. doi: 10.1242/jcs.105171

Fig. 5.

Fig. 5.

APPL1 regulates expression of NF-κB target genes. Analysis of gene expression upon APPL1 knockdown (A) or overexpression (B) in HEK293T cells. A predesigned 96-well array containing primer pairs for detection of 84 genes involved in NF-κB signaling was used. The vertical lines indicate the 1.5-fold change in gene expression threshold. The horizontal line indicates the P = 0.05 of the t-test threshold. Only names of the genes significantly down- or upregulated are mentioned on the charts. Expression of the genes encoding IL-8, TNF, CCL2 and CXCL12 upon APPL1 knockdown (C) or overexpression (D) was validated by qRT-PCR in HEK293T cells 2 days after transfection with non-targeting siRNA (φ) or APPL1 siRNAs (#1 or #2) (C) or APPL1-expressing vector (D). (E) APPL1 level regulates the amounts of secreted IL-8. ELISA analyses of IL-8 production by HEK293T cells 48 h after transfection with non-targeting siRNA (φ), APPL1 siRNA #1 (+) or APPL1-expressing vector.