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. Author manuscript; available in PMC: 2012 Oct 27.
Published in final edited form as: Plant Mol Biol. 2011 Mar 25;76(3-5):211–220. doi: 10.1007/s11103-011-9767-z

Table 2. Aspirational goals for plastid biotechnology.

Examples Future directions
Agronomic importance Except for insect, herbicide and salt tolerance engineered in soybean and carrot, all others traits should be engineered in useful crops. Nitrogen fixation should be first demonstrated in a suitable model plant
Insect resistance
Herbicide resistance
Disease resistance
Drought and salt tolerance
Cold tolerance
Nitrogen fixation
Metabolic pathways Metabolic engineering has been demonstrated in tomato; should be advanced in major crops
Provitamin A
Engineering vitamin B12
Synthesis of long-chain PUFAs
Biopharmaceuticals and vaccines for human health Tobacco is not a suitable system for oral delivery, a major advantage of plant molecular farming. To date, only a few vaccine antigens have been expressed in lettuce chloroplasts. More efforts should be made to express biopharmaceuticals and vaccine antigens in useful crops suitable for oral delivery. Antigen expression in tomato fruit should be improved
Human growth hormones
Human growth factors
Human blood proteins
Vaccines for animal health Tobacco is not suitable for animal feed. More efforts should be made to develop animal vaccines in crops or edible algae which can be used as animal feed
Bacterial antigens
Viral antigens
Phyto-remediation Only phyto-remediation to clean up mercury in soil has been demonstrated so far. Many other toxic metals and persistent organic pollutants should be addressed. Phyto-remediation systems should be developed to clean up water
Heavy metals
Organomercury compounds
Biofuels and industrial enzymes Cocktails for two biomasses (pine wood, citrus peel) were developed; more work is needed for developing new enzymes for several other biomasses. In addition, valuable compounds and secondary metabolites could be engineered
Cell wall degrading enzymes
Biopolymers
Compounds for cosmetic application
Photosynthesis Photosynthetic efficiency in major crops could be improved to enhance food production. Performance of Rubisco under elevated atmospheric CO2 and abiotic stresses could be addressed
Rubisco
C3 and C4 plants