Table 2.

The primers, polymerase chain reaction (PCR) conditions and restriction enzymes used in this study

		Primer pairs	PCR conditions	Restriction enzymes
DNMT1	+32204A/G	5′-AGAACCTGAAAAAGTAAATCCACCG-3′	96°C for 5 min	MspI
		5′-CATGTGATTCACCCGCTTCAG-3′	(94°C for 30 s, 62°C for 30 s, 72°C for 30 s) × 35 cycles
			72°C for 7 min
	+14395A/G	5′-TGTTTCCTTGTTCTCTGACAC-3′	94°C for 5 min	BstEII
		5′-TTGGTTCCCGTTTTCTAGAC-3′	(94°C for 30 s, 62°C for 30 s, 72°C for 30 s) × 30 cycles
			72°C for 5 min
DNMT3B	−579G/T	5′-GAGGTCTCATTATGCCTAGG-3′	96°C for 5 min	PvuII
		5′-GGGAGCTCACCTTCTAGAAA-3′	(96°C for 30 s, 56°C for 30 s, 72°C for 30 s) × 30 cycles
			72°C for 5 min
MTHFR	+677C/T	5′-TGAAGGAGAAGGTGTCTGCGG-3′	94°C for 5 min	HinfI
		5′-AGGACGGTGCGGTGAGAGTG-3′	(96°C for 30 s, 66°C for 30 s,72°C for 30 s) × 30 cycles
			72°C for 5 min
	+1298G/T	5′-CTTTGGGGAGCTGAAGGACTACTAC-3′	96°C for 5 min	MboII
		5′-CACTTTGTGACCATTCCGGTTTG-3′	(96°C for 30 s, 61°C for 30 s, 72°C for 30 s) × 32 cycles
			72°C for 5 min