Fig. 5.

The presence of Pseudomonas aeruginosa in the biofilm-like structures in the airways of BALB/c mice was confirmed by specific peptide nucleic acid–fluorescence in-situ hybridization (PNA–FISH) technique staining P. aeruginosa red. PMNs are stained blue by 4′, 6-diamidino-2-phenylindole (DAPI). (a) (LB) and (c) (SB) show DAPI-stained lungs and (b) (LB) and (d) (SB) the corresponding PNA–FISH-stained lungs obtained at day 1 after infection with P. aeruginosa-containing alginate beads. All magnification ×400.