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Serum were obtained from all mice by cardiac puncture. Concentrations of the polymorphonuclear leucocytes (PMN) mobilizer granulocyte colony-stimulating factor (G-CSF) were determined by enzyme-linked immunosorbent assay (ELISA). G-CSF concentrations were increased significantly in the small beads (SB) group compared to the large beads (LB) group at day 1 (P < 0·0001). No statistically significant differences were observed at other time-points.
