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Western blot analysis using mouse monoclonal and rabbit polyclonal antibodies. HEK293 cells were transiently transfected with pcDNA3 empty vector, pcDNA3-type A VP1 or pcDNA3-type O VP1. 30 μg of protein extracts were loaded to 12% SDS-PAGE and blotted with the indicated hybridoma culture supernatants (A, B) and with 1:3000 diluted rabbit polyclonal antiserum (C). Lane 1, pcDNA3-type A VP1 transfected; lane 2, pcDNA3-type O VP1 transfected; lane 3, pcDNA3 empty vector.
