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. 2012 Oct;31(5):333–339. doi: 10.1089/hyb.2012.0035

FIG. 1.

FIG. 1.

16F7 specifically detects maspin. (A) Lysate samples of COS-1 cells transiently expressing eGFP fusions of all 13 human clade B serpins or vector alone control were equally loaded onto a 12.5% SDS-PAGE and immunoblotted with 16F7. Maspin appears as a ∼60 kDa product due to eGFP tag. (B) The same blots were stripped and reprobed for GFP as a loading control. (C) Lysate samples of MCF10A cells were separated via 12.5% SDS-PAGE and immunoblotted with the indicated monoclonals or isotype control.