FIG. 2.

Human WNT5A hpromoter A and hpromoter B luciferase reporter constructs. Genomic sequences: The included genomic sequences for the p2178 and p1981 constructs are shown. Putative transcription factor binding sites with names are underlined. Some sites are on the opposite strand. Dotted lines indicate the positions of the PCR primers used to generate the luciferase reporter constructs. Exon sequences are shown in bold and the ATG start codons are underlined. Luciferase reporter constructs: The WNT5A upstream sequences are represented by the black line. The numbers are base-pair (bp) upstream from the first nucleotide of the cDNA, indicated with the NCBI accession number. The gray boxes are sequences downstream of the first nucleotide. The indicated HindIII and BglII sites were used for cloning. The hpromoter B constructs include a 412-bp intron, unique to the hpromoter B-derived transcript. The vector sequences are not to scale. Positions of the selected transcription factor binding sites are indicated. A, AP-1; C, CutL1; F, c-Fos; M, c-Myb; N, nuclear factor (NF)-kappaB; P, Pax2; S, Sp1. NF-κB* is the site identified in Ge et al., (2011).