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. 2012 Nov;31(11):1585–1597. doi: 10.1089/dna.2012.1698

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 3. — Transient transfection of hpromoter A and hpromoter B constructs into NIH3T3 and Caco-2 cells. The constructs shown in Figure 2 and the empty vector pGL4-17 were transiently transfected into the cells, along with a Renilla control vector. The ratio of firefly to Renilla luciferase was determined for four replicas and fold-change determined relative to the longest constructs, p2178 and p1981. The mean fold-change for three or four independent transfections is shown. The bars are±S.E.M. The asterisks indicate significance (p<0.05) in comparison to the longest construct.

FIG. 3. — Transient transfection of hpromoter A and hpromoter B constructs into NIH3T3 and Caco-2 cells. The constructs shown in Figure 2 and the empty vector pGL4-17 were transiently transfected into the cells, along with a Renilla control vector. The ratio of firefly to Renilla luciferase was determined for four replicas and fold-change determined relative to the longest constructs, p2178 and p1981. The mean fold-change for three or four independent transfections is shown. The bars are±S.E.M. The asterisks indicate significance (p<0.05) in comparison to the longest construct.