FIG. 4.
Verification and amplification efficiencies of Wnt5a promoter A and B TaqMan primer–probe sets. (A) Purified human and mouse promoter A- and B-specific PCR products were diluted as indicated and used for TaqMan amplification with each primer–probe set. CT (cycle number) values were plotted. Squares are promoter A primer–probes; triangles are promoter B primer–probes. The slopes of the lines were determined and used to calculate the amplification efficiencies shown in (B). (C) DNA agarose gel of mouse and human promoter A and promoter B PCR products, showing the expected product sizes.