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. 2012 Nov;31(11):1585–1597. doi: 10.1089/dna.2012.1698

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 5. — Increase in hpromoter A and hpromoter B activity in response to TNF-alpha. NIH3T3 cells stably transfected with hpromoter A p2178 and hpromoter B p1981 reporter constructs were treated for 6 and 12 h with 5 ng/mL TNF-alpha only (T) and the following inhibitors with (solid bars) and without (patterned bars) TNF-alpha: NF-kappaB inhibitor (JSH-23, 30 μM) (TJ and J), MEK1/2 inhibitor (U0126, 10 μM) (TM and M), p38 inhibitor (SB203850, 10 μM) (TP and P), Jun N-terminal kinase inhibitor (SP600125, 20 μM) (TJk and Jk). Control cells were treated with DMSO (D) (open bar). Cells were collected and assayed for luciferase activity, expressed as relative light units. Bars are±S.E.M. N=4. For statistical analyses, letters are pair-wise comparisons of each value to the DMSO control. Numbers are statistical pair-wise comparisons between TNF-alpha plus inhibitor and the TNF-alpha only values. A different letter or number indicates significance (p<0.05).

FIG. 5. — Increase in hpromoter A and hpromoter B activity in response to TNF-alpha. NIH3T3 cells stably transfected with hpromoter A p2178 and hpromoter B p1981 reporter constructs were treated for 6 and 12 h with 5 ng/mL TNF-alpha only (T) and the following inhibitors with (solid bars) and without (patterned bars) TNF-alpha: NF-kappaB inhibitor (JSH-23, 30 μM) (TJ and J), MEK1/2 inhibitor (U0126, 10 μM) (TM and M), p38 inhibitor (SB203850, 10 μM) (TP and P), Jun N-terminal kinase inhibitor (SP600125, 20 μM) (TJk and Jk). Control cells were treated with DMSO (D) (open bar). Cells were collected and assayed for luciferase activity, expressed as relative light units. Bars are±S.E.M. N=4. For statistical analyses, letters are pair-wise comparisons of each value to the DMSO control. Numbers are statistical pair-wise comparisons between TNF-alpha plus inhibitor and the TNF-alpha only values. A different letter or number indicates significance (p<0.05).