FIG. 6.

qRT-PCR analysis of endogenous mpromoter A, mpromoter B, and mcyclin D1-specific transcripts in TNF-alpha-treated cells. NIH3T3 cells were treated for 6 h with 5 ng/mL TNF-alpha (T), with and without a NF-kappaB inhibitor (JSH-23, 30 μM) (TJ), MEK1/2 inhibitor (U0126, 10 μM) (TM), and p38 inhibitor (SB203850, 10 μM) (TP). Control cells were treated with DMSO (D). The relative level of mpromoter A, mpromoter B, and mcyclin D1 transcripts were determined in comparison to the DMSO-treated cells, using GAPDH as the internal control.