Table 2.
Primer and Probe Sequences for PCR and qRT-PCR
| Primers for Wnt5a reporter constructs | Primer sequences |
|---|---|
| hpromoter A | |
| p2178 | F-GCTAAGCTT/GCGTGTGTGTGTCTTCACCTTG |
| p1707 | F-GCTAAGCTT/CAGTGGGAGCGGTCTGGGATACGC |
| p1358 | F-GCTAAGCTT/GAGGGAGAGAGTAAGGCAGTTGG |
| p773 | F-GCTAAGCTT/CTCACCGCACAGCAATAAGTTCCGG |
| p420 | F-GCTAAGCTT/CGCCAAGAGTTTGCAGAATCTGTGG |
| R-GCTAAGCTT/GAACCGGAGCTGAAGCGGGCACTGGCG | |
| hpromoter B | |
| p1981 | F-GCTAGATCT/TTGCACTGGGATTGAAGAGGGAAGA |
| p1257 | F-GCTAGATCT/TCTTTGAGAAGCCCCGAAGCGTCCA |
| p817 | F-GCTAGATCT/GGAATCGATGCGCCCAGCTGCGGCTCG |
| p356 | F-GCTAGATCT/CCAGTTTCCTTGGTTGGGAGACCCGA |
| R-GCTAGATCT/TTGATTGACTGCGCTTCTCCTCCGT |
| Primers and probes for qRT-PCR | Primer sequences |
|---|---|
| hpromoter A | |
| Forward | TCGGGTGGCGACTTCCT |
| Reverse | CAACTCCTGGGCTTAATATTCCAAT |
| Probe | CGCCCCCTCCCCCTCGCCATGAAG |
| hpromoter B | |
| Forward | CCTCTCGCCCATGGAATT |
| Reverse | GGGCTTAATATTCCAATGGACTTC |
| Probe | CTGGCTCCACTTGTTGCTCGGCC |
| mpromoter A | |
| Forward | GTGGCGACTTCCTCTCCGT |
| Reverse | CGGTCCCCAAAGCCACT |
| Probe | CCCCTCGCCATGAAGAAGCCCA |
| mpromoter B | |
| Forward | ACTTGTTGCTCCGGCCC |
| Reverse | CGGTCCCCAAAGCCACT |
| Probe | AGAAGCCCATTGGAATATTAAGCCCGG |
All primers are shown 5′ to 3′. The diagonal lines demarcate the added extra nucleotides with restriction sites from actual primer sequence. Reverse primers (R) were paired with each of the associated forward (F) primers.