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Expression of rTonB2 (A) and WB analysis of MAbs (B–E). Protein samples were separated by SDS-PAGE and transferred to nitrocellulose membranes, then reacted with MAbs 2F2 (B), 2G8 (C), 3D2 (D), and 6F10 (E), separately. Lane M, pre-stained protein marker; lane 1, E. coli BL21(DE3) cell lysate containing plasmid pET-28a; lane 2, rTonB2.
