Table 2.
Parameters obtained from dynamic force spectroscopy at pH 2.0 in low and high ionic strengths (IS).
| IS (mM) | koff(1)(s−1) | T1 (s) | ΔG (KBT) | x(1)(nm) | koff(2)(s−1) | T2 (s) | ΔG (KBT) | x(2)(nm) |
|---|---|---|---|---|---|---|---|---|
| 10 | 3.3 ± 1.7 | 0.30 | 28.3 | 0.24 ± 0.04 | 62.0 ± 9.4 | 0.02 | 25.3 | 0.04 ± 0.01 |
| 150 | 2.0 ± 0.8 | 0.50 | 28.8 | 0.28 ± 0.03 | 54.0 ± 8.9 | 0.02 | 25.5 | 0.06 ± 0.01 |
koff – dissociation rate constant for the peptide-peptide pair, x – the position of energy barrier. The indices 1 and 2 in the subscript of the parameters refer to the outer and inner barriers of the energy landscape, respectively.