| Reagent | Volume (μl) | Final |
|---|---|---|
| Template, 5 ng μl−1 | 2 | 10 ng |
| Primer mixture, 10 μM | 2 | 0.2 μM |
| dNTP (10 mM, containing varying ratios of dCTP and dhmCTP) |
5 | 1 mM |
| Reaction buffer, 10× | 5 | 1× |
| Taq polymerase (5 U μl−1) | 1 | 0.1 U |
| ddH2O | Up to 50 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.