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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Dermatol Sci. 2012 Sep 7;68(2):99–107. doi: 10.1016/j.jdermsci.2012.09.001

Fig. 4. TSA treatment did not affect the frequencies of Tregs and iNKT cells, as well as the function of iNKT cells.

Fig. 4

(A) Foxp3 expression was detected by intracellular staining. Treg cells were identified as CD4+ Foxp3+ cells. (B) Summary plots showing the frequency of CD4+ Foxp3+ T cells from spleen and lymph node, p>0.05. (C, D) The frequency and absolute number of iNKT cells were shown as α-GalCer-loaded CD1d-tetramer+TCRβ+ lymphocytes gated on B220− cells from spleen and lymph node, p>0.05. (E–H) The production of IL-4 and IFN-γ by iNKT cells in spleen (E&F) and lymph node (G&H) was analyzed with intracellular cytokine staining after PMA/ionomycin stimulation for 3h in vitro. Representative results of three independent experiments are shown. Data are presented as mean ± SD. (N=5, P>0.05).