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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Inorg Biochem. 2011 Sep 22;105(12):1786–1794. doi: 10.1016/j.jinorgbio.2011.09.026

Table 1.

UV-Vis absorption peak positions and postulated heme iron coordination modes in several Tyr- or His-ligated ferrous heme proteins and their ligand complexes

Ferrous
derivative		 Heme protein Soret peak Visible peaks Coordination mode Coordination
number		 Preparation
method		 Ref (s)
Deoxyferrous BLC 411 567 600 Tyr-O-Fe(II) 5 Photoreduction [25]
BLC 413 561 595 Tyr-O-Fe(II) a 5 Photoreduction This study
cAOS 436 558 590 Tyr-O(H)-Fe(II) a 5 Dithionite This study
MAP 440 562 595 Tyr-O(H)-Fe(II) a 5 Dithionite This study
HRP 436 556 580 sh His-N-Fe(II) 5 Dithionite This study b
H93Y Mb (sperm
whale)		 428 556 ( -- ) c Tyr-O(H)-Fe(II) a 5 Dithionite [39]
H93Y Mb (human) 427-429 558-560 (~580sh) His-N-Fe(II) 5 Dithionite [14]
H93G Mb 427-428 558 (~580sh) H2O-Fe(II) 5 Dithionite [43, 45]
Mb (wt) 433 558 (~580sh) His-N-Fe(II) 5 Dithionite [20]
Ferrous-NO BLC nr 559 587 Tyr-O-Fe(II)-NOa 6 H2O2 + azide [36]
BLC nr 555 585 Tyr-O-Fe(II)-NOa 6 H2O2 + azide [40]
BLC 425 556 ~580 sh Tyr-O-Fe(II)-NOa 6 H2O2 + azide [41]
cAOS 426 ~555 bp Tyr-O-Fe(II)-NOa 6 NO + dithionite This study
MAP 403 548 575 Fe(II)-NO 5 NO +
ditdithionite		 This study
Ferrous-CO BLC nr 540 575 Tyr-O-Fe(II)-COa 6 H2O2 + azide + CO [36]
BLC 427 545 580 Tyr-O-Fe(II)-CO 6 H2O2 + azide + CO [42].
BLC 425 541 568 Tyr-O-Fe(II)-COa 6 Photoreduction + CO [25]
BLC 425 544 570 Tyr-O-Fe(II)-COa 6 Photoreduction + CO This study
cAOS 425 541 568 Tyr-O-Fe(II)-COa 6 Dithionite + CO This study
MAP 425 541 568 Tyr-O-Fe(II)-COa 6 Dithionite + CO This study
H93Y Mb 420 539 569 Tyr-O(H)-Fe(II)-CO 6 Dithionite + CO [20]
H93G Mb 418 nr nr H2O-Fe(II)-CO 6 Dithionite + CO [43]
Ferrous-O2 BLC
(compound III)		 414 550 583 Tyr-O-Fe(II)-O2a 6 Compound II + H2O2 [38]
cAOS 415.5 546 580 Tyr-O-Fe(II)-O2a 6 Fe(II) + O2 This study
MAP 422.5 552 584 Tyr-O-Fe(II)-O2a 6 Fe(II) + O2 This study
Mb (sperm
whale)		 418 543 581 His-N-Fe(II)-O2 6 Fe(II) + O2 [28]
a

The identity of the axial ligand, deprotonated (tyrosinate, Tyr-O−) or neutral tyrosine [Tyr-O(H)], was postulated in this study. The axial ligand identity was not explicitly indicated or speculated in the cited references except for “This study”.

b

The spectrum was essentially identical to that reported in refs. [23] and [24].

c

The presence and position of a shoulder are not specifically reported.

nr

Not reported.

sh

Shoulder.

br

Broad peak.