Table I.
Published ζ potentials of plant protoplasts or PM vesicles in various media compared with calculated surface potentials
Species and Ref. | Solution No. | pH | CaCl2 | MgSO4 | NaCl | KCl | LaCl3 | ζ Potential | ψ0,Y | ψ0,A |
---|---|---|---|---|---|---|---|---|---|---|
mm | mV | |||||||||
Barley leaf protoplasts (Abe and Takeda, 1988) | ||||||||||
1 | 6.7 | 0.1 | 0.5 | −48 | −66 | −65 | ||||
2 | 6.7 | 0.1 | 6.5 | −39 | −59 | −58 | ||||
3 | 6.7 | 0.1 | 0.5 | 6.0 | −39 | −59 | −58 | |||
4 | 6.7 | 0.1 | 0.5 | 0.1 | −17 | −7 | −6 | |||
5 | 6.7 | 0.1 | 0.5 | 0.3 | 0 | +2 | +3 | |||
6 | 6.7 | 0.1 | 0.5 | 1 | +14 | +12 | +13 | |||
7 | 3.6 | 0.1 | 6 | −2 | −14 | +5 | ||||
8 | 3.6 | 0.1 | 1 | +23 | +10 | +17 | ||||
Barley leaf protoplasts (Obi et al., 1989a) | ||||||||||
9 | 7.6 | 3 | −29 | −100 | −100 | |||||
10 | 7.6 | 7.5 | −20 | −82 | −82 | |||||
11 | 7.6 | 15 | −13 | −67 | −66 | |||||
12 | 7.6 | 1 | 14 | −13 | −67 | −66 | ||||
13 | 7.6 | 30 | −10 | −52 | −51 | |||||
14 | 7.6 | 1 | 4.5 | −10 | −36 | −36 | ||||
15 | 7.6 | 1a | 4.5 | −11 | −36 | −36 | ||||
16 | 7.2 | 0.5 | 0.17 | 0 | −2 | −2 | ||||
17 | 6.5 | 5 | 1 | +3 | +12 | +12 | ||||
Corn root PM vesicles (Gibrat et al., 1985) | ||||||||||
18 | 6.5 | 15 | −24 | −64 | −64 | |||||
19 | 6.5 | 65 | −14 | −36 | −36 | |||||
20 | 6.5 | 6 | 15 | −8 | −16 | −15 | ||||
21 | 6.5 | 6 | 65 | −6 | −13 | −12 | ||||
Tobacco leaf protoplasts (Nagata and Melchers, 1978) | ||||||||||
22 | 5.8 | 6.7 | 10 | −28 | −56 | −55 | ||||
23 | 5.8 | 1 | 6.7 | 10 | −25 | −32 | −32 | |||
24 | 5.8 | 10 | 6.7 | 10 | −9 | −10 | −9 | |||
25 | 5.8 | 100 | 6.7 | 10 | 0 | +7 | +8 | |||
Rauwolfia serpentina cultured cell protoplasts (Obi et al., 1989b) | ||||||||||
26 | 7.3 | 0.02 | 6 | 1 | −18 | −72 | −71 | |||
27 | 6.3 | 0.02 | 6 | 1 | −18 | −69 | −69 | |||
28 | 5.3 | 0.02 | 6 | 1 | −17 | −56 | −54 | |||
29 | 4.2 | 0.02 | 6 | 1 | −12 | −27 | −18 | |||
30 | 3.0 | 0.02 | 6 | 1 | +2 | −5 | +29 | |||
Barley leaf protoplasts (Obi et al., 1990) | ||||||||||
31 | 7.3 | 0.02 | 6 | 1 | −18 | −72 | −71 | |||
32 | 6.3 | 0.02 | 6 | 1 | −16 | −69 | −69 | |||
33 | 5.2 | 0.02 | 6 | 1 | −12 | −54 | −51 | |||
34 | 4.0 | 0.02 | 6 | 1 | +1 | −22 | −10 | |||
35 | 2.7 | 0.02 | 6 | 1 | +18 | −2 | +40 |
ψ0,Y is the potential computed by the model of Yermiyahu et al. (1997c) and ψ0,A is the potential computed by the adjusted model of the present study. For each of the two models, a single set of parameter values was used throughout. The column designated NaCl includes NaCl plus any monovalent buffer ions.
MgCl2.