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. 2012 Oct 23;3(5):e00372-12. doi: 10.1128/mBio.00372-12

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Copyright © 2012 Porsch et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 2 — Knh is an outer membrane protein required for full-level adherence to Chang human epithelial cells. (A) Western blot analysis of outer membrane preparations from K. kingae strains KK03 (WT), KK03pilA1, KK03knh, and KK03knh/(knh) under standard SDS-PAGE denaturing conditions. (B) Western blot analysis of formic acid-treated outer membrane preparations from K. kingae strains KK03 (WT), KK03pilA1, KK03knh, and KK03knh/(knh) under standard SDS-PAGE denaturing conditions. Molecular sizes (MW) are expressed in kilodaltons. (C) Adherence of K. kingae strains KK03 (WT), KK03pilA1, KK03knh, and KK03knh/(knh) to Chang human epithelial cells. Adherence assays were conducted in triplicate. Error bars indicate standard errors of the means. Statistical analyses were performed by using the unpaired t test to compare the adherence levels of two strains as follows: P < 0.05 (*) for KK03 versus KK03knh, KK03pilA1 versus KK03knh, and KK03knh versus KK03knh/(knh).