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. Author manuscript; available in PMC: 2013 Jul 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Jun 5;51(27):6717–6721. doi: 10.1002/anie.201202204

Figure 2.

Figure 2

Employing a pair of signalling probes differing in affinity we can broaden the dynamic range of E-DNA sensors. (A) We did so by co-immobilizing (1:1 ratio) on a single electrode surface a relatively low affinity E-DNA probe (e.g., probe 3GC, Kd = 580 nM) with a higher affinity E-DNA probe (e.g., probe 0GC, Kd = 19 nM). (B) The useful dynamic range (defined as the fold-concentration change upon transition from 10% occupancy to 90% occupancy) of these individual probes spans an 81-fold range of target concentrations over two distinct concentration regimes. (C) With this strategy the resulting dose-response curve is extended and spans a 1,000-fold range of target concentrations.