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. 2012 Jul 28;12:127. doi: 10.1186/1471-2148-12-127

Table 2.

Activity of newly identified specifier proteins

 
Incubation with myrosinase and allylglucosinolate
Incubation with myrosinase and benzylglucosinolate
  Thiocyanate Epithionitrile Simple nitrile Thiocyanate Simple nitrile
ApTFP1
+
+
-
-
+
ChESP1
-
+
-
-
+
CiESP1
-
+
-
-
+
DaESP1
-
+
-
-
+
DlESP1
-
+
-
-
+
ItESP1
-
+
-
-
+
SpESP1
-
+
-
-
+
ChNSP1
-
-
+
-
+
ItNSP1
-
-
+
-
+
SpNSP1 - - + - +

Activity was tested using crude extracts of bacteria expressing specifier protein cDNAs, 1 mM allyl- or 2 mM benzylglucosinolate and 0.005 units myrosinase in 50 mM MES buffer, pH 6.0. The presence of non-isothiocyanate products (organic thiocyante, epithionitrile, simple nitrile) in dichloromethane extracts of the assay mixtures after 30 min incubation is indicated by +, the absence by - (in comparison to assays with extracts of bacteria harbouring the expression vector without cDNA insert, for chromatograms see Additional file 5: Figure S3).