Figure 3.

Determination of enzyme kinetic parameters, K_M and k_cat. Comparison of on-chip and off-chip results indicates no need of inner filter effect correction factor for on-chip data. The off-chip velocities, after applying correction factor, shows marked improvement in the velocities at each substrate concentration. The error bar is obtained from three individual experiments. The exponential nature of corrector factor increases the magnitude of error bar, especially at higher substrate concentrations in corrected off-chip velocity data.