Figure 5.
Quantitative Analysis of Proteome in Proliferating Cells
(A) Abundance distribution for mRNAs (green) and proteins (red). Red vertical lines delimit expression zone 2 (0.5–2 mRNA copies/cell). See also Figures S2, S3, and Table S10.
(B) Absolute abundance for all mRNAs (each dot represents a gene). Dark and light blue dots correspond to genes for which proteins were detected or not, respectively.
(C) Protein versus mRNA abundance. Black curve, sliding median.
(D) Protein/mRNA ratio versus protein abundance. Red dots: ribosomal proteins; black curve: sliding median.
(E) Protein abundance for selected functional categories. Each dot represents a protein. Haploid Schizosaccharomyces pombe cells contain 5,110 and 10,220 annotated protein-coding genes in G1 and G2 phase, respectively (red zone), and 5,348 introns across 2,523 intron-containing genes (red and yellow zones, respectively). In proliferating cells, we measured ∼41,000 mRNA molecules (dark green line) and 1.1–2.6 × 105 copies of each rRNA (green zone). Ribosomal proteins copies/cell for paralogs were summed up.
See also Figure S6.