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. 2012 Oct 26;151(3):671–683. doi: 10.1016/j.cell.2012.09.019

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© 2012 ELL & Excerpta Medica.

Open Access under CC BY 3.0 license

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Figure S3 — Technical and Biological Variability in Proteomics Data, Related to Figure 5

(A) Technical variability determined from replicate LC-MS/MS analyses for MM samples (proliferating). The median, mean, lower and upper endpoints of 95% confidence interval (L95 and U95) are displayed for proteins being quantified by 1, 2, 3 or more peptides.

(B) Expression variability (technical and biological) between the three MM biological replicates (proliferating).

(C) Technical variability determined from replicate LC-MS/MS analyses for MN samples (quiescent). The median, mean, lower and upper endpoints of 95% confidence interval (L95 and U95) are displayed for proteins being quantified by 1, 2, 3 or more peptides.

(D) Expression variability (technical and biological) between the three MN biological replicates (quiescent).

(E) Hierarchical clustering of absolute protein abundance in copies per cell (log10) for all 6 samples (3x MM, 3x MN) measured in duplicates each. The column dendrogram representing the clustering of the different samples is displayed.