Figure 2.

Number of colony-forming units (CFU) in the lung and spleen and correlations of CFU, CD4⁺/CD4⁺ Foxp3⁺ ratio and CD4⁺ Foxp3⁺ cells from immunized mice. Different groups of mice were challenged with 1 × 10⁵ bacilli of Mycobacterium tuberculosis 30 days after immunization (a). Lung and spleen CFU counts were evaluated on day 30 post challenge (b). The total number of lung CD4⁺ (c) and total number of lung CD4⁺ Foxp3⁺ cells (d) are shown. The ratio of CD4⁺/CD4⁺ Foxp3⁺ cells (e), the ratio of lung CFU counts and CD4⁺/CD4⁺ Foxp3⁺ cells (f), the ratio of lung CFU counts and total number of CD4⁺Foxp3⁺ cells (g) are shown. The cell number is expressed as the mean ± SEM for individual animals. The CFU counts are expressed as Log₁₀ of the mean ± SEM for individual animals. The data are representative of two independent experiments (DNA-heat-schock protein (hsp) 65, 7; BCG/DNA-hsp 65, 7; BCG/CFP-CpG, 8; pVAX, 4; BCG, 7). *P < 0·05 compared with non-immunized and infected mice (black dotted line); &P < 0·05 compared with non-immunized and infected mice (grey dotted line); #P < 0·05 compared with non-immunized, uninfected mice (solid line). Horizontal bars represent the significant difference between groups.