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. 2012 Nov;23(11):1835–1846. doi: 10.1681/ASN.2012010022

Figure 3.

Figure 3.

LXR agonist suppresses diabetes-induced OPN mRNA and protein expressions in the kidney. (A) Total RNA was isolated from the kidney samples and analyzed for the OPN mRNA expression levels by qRT-PCR. Renal OPN mRNA expression is significantly increased in the DM group compared with the control group. The OPN expression was normalized by the GAPDH expression. Data are means ± SEM. *P<0.05. (B) Localization of renal OPN protein by immunohistochemistry. OPN protein is predominantly localized in the interstitium of the kidney in the DM group, and its expression is suppressed in the DM+T0901317 group compared with the DM group. Original magnification, ×400. (C) Western blotting analysis of OPN protein expression. Full-length OPN as well as the C-terminal fragment of MMP-cleaved OPN are significantly upregulated in the DM group compared with the control group, and they are suppressed in the DM+T0901317 group compared with the DM group. Quantification was performed by densitometry of three independently performed experiments, with normalization by β-actin. Data are means ± SEM. *P<0.05.