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Cryoglobulin activity was conserved in more galactosylated X10C and 6-19.ST6-5 variants, but lost in the highly sialylated 6-19 F243A mutant. The amounts of cryoprecipitates were estimated by measuring OD280 of supernatants, and results are expressed as percentages of cryoprecipitation (means of duplicates).
