Figure 3. Batf compensates for CD8α+ cDC development in Batf3−/− mice.
a, Wild type (WT), or Batf3−/− (BATF3KO) BM cells were infected with GFP-RV33 (Empty) or retrovirus expressing the indicated cDNA and cultured with Flt3L7. Histograms for the indicated markers are for B220−CD11c+ cells on day 10. Numbers are the percent of cells in the gate. b, Inguinal lymph nodes from WT, Batf3−/− (BATF3 KO) or Batf−/−Batf3−/− mice (BATF1/3 DKO) on 129SvEv or C57BL/6 backgrounds were analyzed by FACS. Shown are histograms for DEC205 and CD8α. c, CD4 T cells of the indicated genotype were differentiated twice under TH2 conditions5 and analyzed by FACS for intracellular IL-10.