Expression Pim kinase protein in cell lines and lymphoma patient tissue samples. (A) Pim-1, -2, and -3 expressions in JeKo-1 and Mino cells. Cells were either untreated or treated with DMSO, and immunoblots were prepared for Pim protein expressions. Note: multiple bands in Pim-1 and Pim-2 are expected because of multiple translation initiation sites.25 (B) Histologic section of case of MCL stained with hematoxylin and eosin, and immunohistochemical staining of MCL patient tissue samples, stained with rabbit anti–Pim-1, -2, and -3 antibodies and counterstained with hematoxylin.