Table 1.

Analysis of 3′HS1 and 3′HS2 function in transgenic mice

Construct	Line	Copy no.	huβ-mRNA/mα-mRNA					huβ-mRNA/copy*	Uniform, variegated
β-globin	A	1	0.001 + 0.0005†					0.001	ND
	B	2	0.0025 + 0.002†					0.001	ND
	C	1	0.002 + 0.001†					0.002	ND
	D	1	0.001 + 0.0001†					0.001	ND
	E	3	0.000 + 0.0003†					0.000	ND
	F	1	0.066 + 0.012					0.066	U
	G	1	0.001 + 0.0001†					0.001	ND
	H	2	0.002 + 0.001†					0.001	ND
	I	1	0.001 + 0.0003†					0.001	ND
	J	1	0.002 + 0.0005†					0.002	ND
Mean: 0.008 + 0.02
3′HS1-β-globin-3′HS1	A	10	2.47 + 0.46					0.247	U
	B	1	0.17 + 0.04					0.17	U
	C	4	0.22 + 0.024					0.06	V
	D	3	0.23 + 0.012					0.078	V
	E	3	0.37 + 0.02					0.12	U
	F	4	2.26 + 0.17					0.565	V
Mean: 0.21 + 0.19 P = 0.009‡
3′HS2-β-globin-3′HS2	A	1	0.033 + 0.005					0.033	U
	B	1	0.011 + 0.002					0.011	V
	C	1	0.002 + 0.001†					0.002	ND
	D	2	0.028 + 0.01					0.014	U
	E	2	0.018 + 0.002					0.009	ND
	F	1	0.047 + 0.012					0.047	V
Mean: 0.02 + 0.02 P = 0.186‡
3′HS1 + 2-β-globin-3′HS1 + 2	A	4	0.127 + 0.034					0.032	U
	B	5	0.05 + 0.008					0.01	ND
	C	2	0.083 + 0.012					0.042	U
	D	3	0.012 + 0.006					0.004	ND
	E	6	0.192 + 0.055					0.032	U
	F	1	0.053 + 0.013					0.053	ND
	G	1	0.037 + 0.013					0.037	ND
	H	3	0.147 + 0.062					0.049	U
	I	9	0.22 + 0.037					0.025	U
	J	5	0.13 + 0.016					0.026	U
Mean: 0.031 + 0.016

U indicates uniform expression (100% of cells express human β-globin); V, variegated expression (< 100% of cells express human β-globin); and ND, level of human β-globin protein was insufficient for analysis.

^*huβ-mRNA/copy = huβ-mRNA/mα-mRNA divided by copy number for each line.

^†mRNA levels below 0.005 are indistinguishable from background and are scored as negative.

^‡Compared with 3′HS1 + 2-β-globin-3′HS1 + 2.